Journal: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology

Article Title: High throughput toxicity screening and intracellular detection of nanomaterials

doi: 10.1002/wnan.1413

Figure Lengend Snippet: Advantages and Limitations of High Throughput Screening Methods to Study Toxicity of Nanomaterials

Article Snippet: A microfluidic chip‐based IFC developed by Amphasys AG (Switzerland) can analyze single cells without any specific sample preparation prior to measurement., , Compared to other impedance‐based cytometers, e.g., Z series Coulter Counters or the CASY from Roche, the microfluidic chip‐based IFC can cover impedance measurements at a broader frequency range, and thus yield information regarding the size and number of cells and, in addition, their membrane capacitance and cytoplasmic conductivity., IFC gives a snapshot of the cellular state of single cells based on the changed resistance within the chip‐channel caused by the passing cells.

Techniques: High Throughput Screening Assay, Flow Cytometry, Multiplexing, Fluorescence, Imaging, Biomarker Discovery, Spectroscopy, Cell Culture, Battery, Single Particle, Concentration Assay, Clinical Proteomics, Membrane, Single Photon Emission Computed Tomography, Marker, In Vitro, In Vivo, High Content Screening, Multiplex Assay, Suspension, End Point Assay, Gene Expression, Amplification, Microarray, Single Cell Gel Electrophoresis, Staining

Journal: Light, Science & Applications

Article Title: Optoacoustic microscopy at multiple discrete frequencies

doi: 10.1038/s41377-018-0101-2

Figure Lengend Snippet: a A scheme of the µDoppler detection set-up. FL1 − flow 1 away from the US sensor, FL2 − flow 2 away from the US sensor (FL2 − < FL1 − ), FL1 + flow 1 toward the US sensor, IN flow direction into the chip, MC microfluidic chip, OL objective lens, P particles, US ultrasound, UT ultrasound transducer, f mod modulation frequency, OUT flow direction out of the chip. The close-up views illustrate the experimental detection of particles moving away from the ultrasound sensor, which is equivalent to a Doppler red shift. b - d Averaged frequency spectra acquired at flow speeds of 0 mm·s −1 (green), 0.3 mm·s −1 (red), or 1.3 mm·s −1 (red). The latter two flow speeds show respective red shifts of 2 Hz and 7 Hz from the modulation frequency because particles are flowing away from the transducer. e Doppler shifts measured from carbon particles as a function of the flow speed in a microfluidic chip. The black line shows a linear fit to the data. f A maximum intensity projection of a region of interest (ROI) of size 160 × 160 µm² in the mouse ear, which shows micro-vascularization. Scale bar, 30 µm. g A Doppler FDOM-flow map that was recorded in the same ROI, showing a peak amplitude of the recorded flow in the blood vessels. h , i A blend and an overlay of the Doppler flow map g and the optoacoustic image f, which show peak amplitudes as Doppler red and blue shifts relative to the transducer position. j An overlay of Doppler red- and blue-shift maps on the galvanometric scan in panel f. White arrows indicate the inferred directions of blood flow in various vessels. k A profile scan across a single capillary at the position indicated by the white arrows in the galvanometric scan in panel g. The red line represents a parabolic fit to the recorded Doppler shift data with a maximum blood flow speed of 0.44 mm·s −1 . The gray solid curve shows the peak amplitudes at each measurement position

Article Snippet: The particle suspension was pumped at known speeds through a microfluidic chip (product no. 01-0175-0138-02, microfluidic ChipShop, Jena, Germany; channel cross-section, 1 mm × 200 μm; lid, 140 μm TOPAS) using a syringe pump (540060, TSE systems, Bad Homburg, Germany).

Techniques: